SIMPLE STAINING
Simple Staining Simple staining is used to stain prokaryotic cells, e.g., microorganisms, to determine the shape, size, and arrangements. In this staining method, only a single dye is needed. It’s simple with the procedure which includes covering fixed smears with stain for a minute, and excess stain is washed off with water and blotted dry. In this case, basic dyes are used which are methylene blue, crystal violet, and carbolfuchsin. (a) Crystal violet stain of Escherichia coli(b) Methylene blue stain of Corynebacterium