Micro-Pipette: Types, Uses, and Handling Techniques in the Laboratory

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Micro-Pipette: Types, Uses, and Handling Techniques in the Laboratory

Micro-Pipette

A micropipette is an essential laboratory instrument used for accurately measuring and transferring small volumes 1ml or less than 1ml of liquid. The three important parts of a micropipette are the plunger button, the volume adjustment dial, and the disposable tip.

  • Plunger Button:
    The plunger button is pressed to aspirate (draw in) and dispense (release) the liquid. It is important to press the plunger button smoothly and steadily to avoid introducing air bubbles into the liquid.
  • Volume Adjustment Dial:
    This part allows users to set the desired volume for aspiration and dispensing. It is crucial to set the volume accurately according to the requirements of the experiment.
  • Disposable Tip:
    The tip is a crucial part of the micropipette. It comes in various sizes and should be selected based on the volume to be measured. Tips are disposable and should be changed for each new sample to prevent cross-contamination.

Uses of Micro Pipettes:

Micropipettes are commonly used in various scientific and medical applications, including:

  • Molecular Biology:
    Used for DNA, RNA, and protein analysis.
  • Clinical Diagnostics:
    Used for handling small volumes of blood, serum, or other biological fluids.
  • Microbiology:
    Used for culturing and transferring small volumes of microbial samples.
  • Chemistry:
    Used for preparing solutions and measuring reagents.
  • Caring for Micro Pipettes:
    Proper care is essential to maintain the accuracy and longevity of micropipettes:

Calibration:

Regularly calibrate the micropipette to ensure accurate volume measurements.

  •  Tip Usage:
    Use high quality, compatible tips, and change them for each new sample.
  • Cleaning:
    Clean the external surfaces regularly with a mild detergent or disinfectant.
  • Avoid Overloading:
    Do not exceed the maximum volume limit specified for the micropipette.

Handle with Care: Avoid dropping or banging the pipette, as it can affect its accuracy.

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