CALCIUM (Arsenazo III) TEST KIT (MONOTEST)
INTENDED USE:
Intended for in vitro quantitative determination of Calcium in Serum.
PRINCIPLE
Calcium forms a purple complex with Arsenazo III at neutral pH. Intensity of colour is proportional to calcium concentration and is measured photometrically.
Calcium + Arsenazo III → Blue-purple complex
CONTENTS
Reagent 1: Calcium (ASIII) Reagent – 2 x 15 mL
Reagent 2: Calcium Standard – 1 x 1 mL SAMPLE COLLECTION & PRESERVATION
Use serum.
Calcium is stable 7 days at 2–8°C when stored in tightly closed glass vials.
Avoid haemolysed specimens.
REAGENT PREPARATION & STORAGE
Store at 2–8°C.
Stability up to expiry date when stored properly.
Protect from direct light.
Do not freeze.
PROCEDURE
Label test tubes: Blank (B), Standard (S), Sample (T)
Add reagents:
Reagent 1: 1000 µL to all
Standard: 10 µL to S
Sample: 10 µL to T
Mix and incubate for 5 minutes at 37°C
Measure absorbance of S and T against reagent blank at 650 nm
CALCULATION
Calcium (mg/dL) = (Absorbance of Test / Absorbance of Standard) × 10
NORMAL VALUES
Serum calcium: 8.5 – 10.5 mg/dL
(Values may vary by population and methodology)
CLINICAL SIGNIFICANCE
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Calcium is found mainly in the bones (approx. 98%). In serum, calcium exists in three forms:
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Protein-bound
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Ionized (physiologically active)
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Complexed with anions
Serum calcium levels may increase in:
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Hyperparathyroidism
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Hyperthyroidism
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Excess Vitamin D
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Malignancy
Levels may decrease in:
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Hypoparathyroidism
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Renal failure
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Vitamin D deficiency
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Pancreatitis
GENERAL SYSTEM PARAMETERS
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Reaction type: End point
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Wavelength: 650 nm (600–660 nm)
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Reaction temperature: 37°C
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Sample volume: 10 µL
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Reagent volume: 1000 µL
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Standard: 1 mmol/L
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Incubation: 5 minutes
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Read against reagent blank
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LINEARITY
Linear up to 15 mg/dL.
If sample >15 mg/dL, dilute and multiply result by dilution factor.
NOTE
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Reagent is very stable; colour intensity develops uniformly.
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Avoid contaminated glassware.
LABELING
Code No. 317, Pack Size 2 × 15 mL, Standard 1 mL
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