HISS’S STAIN

INTRODUCTIONOF CULTURE MEDIA Most bacteria can be cultured artificially on culture media containing required nutrients,

“Microbiology Worlds” Microbiology is the scientific study of microorganisms, which are microscopic living organisms that

Bacteria:- Bacteria are single-celled microorganisms that have a relatively simple structure compared to other living

ACID FAST STAIN:- • A few types of bacteria, such as the mycobacteria and Nocardia

CALCIUM (Arsenazo III) TEST KIT (MONOTEST)*BEACON INTENDED USE : This reagent is intended for the

AMMONIA KIT (Kinetic Method) INTENDED USE The reagent kit is intended for the in vitro

DEMONSTRATION OF BACTERIAL CAPSULES BY

HISS’S STAINING TECHNIQUE

Reagents-

1. 1% (w/v) crystal violet.

2 20% (w/v) copper sulfate.

Procedure-

1. Mix the organism suspension with one drop of normal saline (0.85 g/dl sodium chloride) (This helps to show the capsules).

2 . Prepare dry smear by using alcohol.

3 . Cover the smear with crystal violet stain and heat gently until steam begins to rise. Leave to stain for one minute.

4 . Wash off the stain with copper sulfate solution.

5. Dry the smear in air.

6. Examine the smear under oil immersion objective.

Result-

1. Bacterial Dark purple

2. Capsule outline Pale blue

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