Improved Neubauer’s Chamber: How It Works, Grid Design & Clinical Use

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Improved Neubauer’s Chamber

Improved Neubauer’s Chamber: How It Works, Grid Design & Clinical Use

A Neubauer chamber is an important tool in the field of hematology and cell biology. It is a specialized counting chamber designed for the manual counting of cells, such as blood cells or sperm cells, under a microscope. Here are some key aspects related to Neubauer chambers:

  • Grids:
    The Neubauer chamber has a central counting area and four corner counting area divided into grids. These grids help in systematic counting of cells.
  • Cover Slip:
    The counting chamber is covered with a glass coverslip, which is essential for creating a consistent depth for the cells to be examined.
  • Chamber Depth:
    The chamber has a specific depth 0.1mm that allows for an accurate volume of the sample to be loaded.
  • Uses:
    Neubauer chambers are commonly used for manual cell counting, such as red and white blood cells, sperm cells, or any other cells present in a liquid sample.Concentration Calculation:

Cleanliness:

Keep the Neubauer chamber and coverslip clean and free from dust or debris. Clean them with a mild detergent and rinse thoroughly with distilled water.

 

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