MICROBIOLOGY INDEX
1. Safety measures in the Microbiology Laboratory working area.
2. Isolation of bacteria
3. Culture media
a. Introduction and preparation technique of Culture Media
b. Type of Culture Media
Nutrient Agar
MacConkey Agar
Blood Agar
EMB Agar
XLD Agar
Deoxycholate Citrate Agar
Lowenstein Jensen Acid Medium
4. Isolation of Bacteria
a. Inoculation Technique
Streaking Method,
Pouring Method
Spreading Method
Lawn Method
b. Incubation of micro-organisms on or in Culture media
c. Study of Bacterial Colony morphology
d. Biochemical identification of microbiology
i. Catalase Test
ii. Coagulase Test
iii. Oxidase Test
iv. Urease Test
v. Indole Test
vi. Bile Solubility test
vii. Cetrimide test
viii. Decarboxylase test.
e. Slide preparation of various Specimens
f. Staining Technique
i. Simple Stain
ii. Gram’s Stain
iii. AFB or ZN Stain
iv. Albert Stain
v. Negative Stain
5. Cultivation of Fungi
6. Motility Testing of micro-organisms
7. Anti-bacterial sensitivity testing
8. Preparation of VDRL buffer and Antigen emulsion
Serology practical index
1. VDRL Agglutination test for syphilis by Quantitative and Qualitative method
2. Rapid Plasma Reagin (RPR) test for syphilis
3. Widal test by Qualitative and quantitative method.
4. Ring Test
5. Nepier Aldehyde Test
6. ASO test by Quantitative and Qualitative
7. Rheumatoid Arthritis Test (RA Test)
8. C-Reactive Protein Test (CRP)
9. Immunologic Pregnancy Test