NEGATIVE STAINING

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NEGATIVE STAINING

DEMONSTRATION OF BACTERIAL CAPSULES BY NEGATIVE STAINING METHOD

Reagents –
10 gm/dl nigrosine solution.

Sample –
A given culture.


Procedure –

  1. Transfer aseptically a loopful of culture on a clean and dry slide.

  2. Mix it with a loopful of stain.

  3. Make a smear by using a glass slide.

  4. Allow it to dry slowly at room temperature (25°C ± 5°C).

  5. Observe under oil immersion lens.


Results –

 

ObservationAppearance
BackgroundBluish black
OrganismsColorless (hollow bodies)

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