Sodium Reagent Kit (Mono Test)
Colorimetric Method
INTENDED USE:
This reagent kit is intended for the in vitro quantitative determination of sodium in serum.
PRINCIPLE:
The reagent is based on reaction of sodium with a selective chromophore producing a chromogenic complex whose absorbance is directly proportional to sodium concentration in the sample.
CONTENTS:
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Reagent 1: Sodium Reagent
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Reagent 2: Sodium Standard (150 mEq/L)
SAMPLE COLLECTION AND PRESERVATION:
Serum or heparinized plasma.
Sodium is stable for 2 weeks at 2–8°C.
REAGENT PREPARATION AND STORAGE:
All reagents are ready to use. Store reagents at room temperature (2–30°C).
PROCEDURE:-
Pipette into clean dry test tubes labeled as Blank (B), Standard (S), and Test (T):
| Component | Blank (B) | Standard (S) | Test (T) |
|---|---|---|---|
| Sodium reagent | 1.0 ml | 1.0 ml | 1.0 ml |
| Standard | — | 10 µl | — |
| Sample | — | — | 10 µl |
Mix well and incubate at RT for 5 minutes.
Measure the absorbance of Standard (Abs S) and Test (Abs T) against reagent blank at 630 nm.
CALCULATION:
Concentration of Sodium (mEq/L) = (Abs T / Abs S) × 150
NORMAL VALUES:
Serum/Plasma: 135 – 155 mEq/L
It is recommended that each laboratory establish its own normal range depending on patient population.
QUALITY CONTROL:
It is recommended that controls be included in each set of assays.
GENERAL SYSTEM PARAMETERS:
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Reaction type: End point
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Wavelength: 630 nm
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Cuvette: 1 cm
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Reaction temperature: Room temperature
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Reagent volume: 1.0 ml
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Sample volume: 10 µl
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Incubation time: 5 minutes
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Blank absorbance limit: ≤ 1.2
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Standard: 150 mEq/L