SIMPLE STAINING

INTRODUCTIONOF CULTURE MEDIA Most bacteria can be cultured artificially on culture media containing required nutrients,

“Microbiology Worlds” Microbiology is the scientific study of microorganisms, which are microscopic living organisms that

Bacteria:- Bacteria are single-celled microorganisms that have a relatively simple structure compared to other living

ACID FAST STAIN:- • A few types of bacteria, such as the mycobacteria and Nocardia

CALCIUM (Arsenazo III) TEST KIT (MONOTEST)*BEACON INTENDED USE : This reagent is intended for the

AMMONIA KIT (Kinetic Method) INTENDED USE The reagent kit is intended for the in vitro

Simple staining is used to stain prokaryotic cells, e.g., microorganisms, to determine the shape, size, and arrangements.
In this staining method, only a single dye is needed.
It’s simple with the procedure which includes covering fixed smears with stain for a minute, and excess stain is washed off with water and blotted dry.
In this case, basic dyes are used which are methylene blue, crystal violet, and carbolfuchsin.

(a) Crystal violet stain of Escherichia coli
(b) Methylene blue stain of Corynebacterium

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