HISS’S STAIN

Introduction:- It is a process by which the cells or tissues are fixed in chemical

INTRODUCTION:- In the laboratory setting, numerous histological specimens are received throughout the day for testing.

Introduction:- Microscopes are instruments designed to produce magnified visual or photographic images of objects too

Introduction:- Microscopes are instruments designed to produce magnified visual or photographic images of objects too

Introduction:- Surgical pathology includes gross and microscopic examination of resected specimens and biopsies by histopathologists

ABO AND Rh TYPING IS PERFORMED BY TWO METHOD:- 1.SLIDE METHOD:- Aim: – ABO grouping

DEMONSTRATION OF BACTERIAL CAPSULES BY

HISS’S STAINING TECHNIQUE

Reagents-

1. 1% (w/v) crystal violet.

2 20% (w/v) copper sulfate.

Procedure-

1. Mix the organism suspension with one drop of normal saline (0.85 g/dl sodium chloride) (This helps to show the capsules).

2 . Prepare dry smear by using alcohol.

3 . Cover the smear with crystal violet stain and heat gently until steam begins to rise. Leave to stain for one minute.

4 . Wash off the stain with copper sulfate solution.

5. Dry the smear in air.

6. Examine the smear under oil immersion objective.

Result-

1. Bacterial Dark purple

2. Capsule outline Pale blue

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