SUMMARY:-

Accurate measurement of glucose in body fluids is important in the diagnosis and management of diabetes, hyperglycemia, adrenal dysfunction, and various other conditions.

Aim: – Estimations of blood sugar by GOD & POD method.

PRINCIPLE:-

Glucose oxidase (GOD) oxidizes the specific substrate β-D- glucose to gluconic acid and hydrogen
peroxide (H2O2) is liberated. Peroxidase (POD) enzyme acts on hydrogen peroxide to liberate nascent oxygen (O2), then nascent oxygen couples with 4- 4-amino antipyrine and phenol to form red quinoneimine dye.