Blood Sugar/Glucose Test
SUMMARY:-
Accurate measurement of glucose in body fluids is important in the diagnosis and management of diabetes, hyperglycemia, adrenal dysfunction, and various other conditions.
Aim: – Estimations of blood sugar by GOD & POD method.
PRINCIPLE:-
Glucose oxidase (GOD) oxidizes the specific substrate β-D- glucose to gluconic acid and hydrogen
peroxide (H2O2) is liberated. Peroxidase (POD) enzyme acts on hydrogen peroxide to liberate nascent oxygen (O2), then nascent oxygen couples with 4- 4-amino antipyrine and phenol to form red quinoneimine dye.
Glucose +H2O GOD Gluconic Acid + H₂O₂
Phenol + H₂O₂ + 4 AAP POD Colored Compound(Quinonimine dye) + H₂O.
Requirment:-
Three test-tube, Calorimeter, Glucose reagent, Distilled water, Incubator, Cuvette, Pipette , etc.
Plasma specimen (in sodium floride vails.)
1. Measure all content according to the chart in three test tubes.mark as blank standered and test.
2.Mix well and incubate for 15 minutes at 37°C temperature in incubator.
3.Read the optical density of the test and standered against blank at 520 nm wave length.
Calculation:-
Blood sugar(mg/dl) = optical density of Test × Consentration of standered optical density of standered
Consentration of glucose = 100 mg/dl
After testing:-
Optical density of Test = 0.23
Optical density of standered = 0.22
THEN,
= 0.23 × 100 0.22
= 104.54
Results:- 104.54 mg/dl
Normal value:-
F.B.S = 70-110 mg/dl
R.B.S = 70-120 mg/dl
p.p = 80 -140 mg/dl
Clinical significance :-
1. Increased sugar level cause hyperglycemia.
2. Decreased sugar level cause hypoglycemia.