INTENDED USE :

This reagent is intended for the in vitro quantitative determination of Calcium in Serum.

CLINICAL SIGNIFICANCE :

Calcium, in the skeleton, is found mainly in the bones (approximately 99%). In the adult, calcium is found in the bones and serum and is bound to serum albumin. Therefore, decreased Albumin causes lower serum calcium levels. Hence, low levels of Calcium in serum are observed in hypoalbuminemia. Increased Calcium levels cause cardiac arrhythmias, renal and biliary calculi and may also be found in bone demineralization, hyperparathyroidism, Vitamin D intoxication, Multiple myeloma, sarcoidosis.
In deficiency, Rickets, Vitamins D deficiency and pancreatitis.

PRINCIPLE :

Calcium ions specifically react with Arsenazo III at a neutral pH to form a blue purple complex. The intensity of the colour is directly proportional to the amount of calcium present in the sample.

Abs. ↑ ∝ Calcium Concentration
Calcium + Arsenazo III → Blue Purple Coloured Complex

CONTENTS :

Reagent 1: Calcium (Stabil) Reagent
Reagent 2: Calcium Standard 10 mg/dL

SAMPLE COLLECTION AND PRESERVATION :

Serum / Heparinized plasma
Stable for 7 days when stored at 2–8°C.

REAGENT PREPARATION AND STORAGE :

The reagents are ready to use.
Once opened, the reagents are stable for 7 days when stored at 2–8°C.
Do not freeze. Protect from direct light.

PROCEDURE :

Label tubes as Blank (B), Standard (S), Test (T).

Mix and read absorbance at 650 nm (Abs.S, Abs.T).

CALCULATION :

Calcium in mg/dL =

NORMAL VALUES :

It is recommended that each laboratory establish its own reference ranges.

• Serum (Adult): 8.7–10.4 mg/dL

• Serum (Children): 8.8–10.8 mg/dL

• LINEARITY :

  
  

  This method is linear up to 15 mg/dL.
  If values exceed this limit, dilute sample 1:1 with saline and repeat the assay.

• GENERAL SYSTEM PARAMETERS

  • Wavelength : 650 nm (620–660 nm)

  • Cuvette path length : 1 cm

  • Temperature : 37°C

  • Sample – 10 µL

  • Reagent 1 – 1000 µL

  • Reagent 2 – 100 µL

  • Mix & read after 1 minute

  • NOTICE :

    This product is a very widely distributed IVD, even outside restricted areas. It does not contain any hazardous substances above 0.1% w/w.
    Packaging waste is recyclable and can be disposed of according to local regulations.

  • ORDERING INFORMATION :

    Test	Pack Size	Reagent 1	Reagent 2
    522A	25 Tests	3 × 10 mL	1 × 3 mL
    522A	100 Tests	3 × 17 mL	1 × 10 mL