CREATININE(Modified Jaffe’s Liquid Reagent):-
INTENDED USE
The reagent kit is intended for the in-vitro quantitative determination of creatinine in serum and urine.
SUMMARY
Creatinine is excreted as a waste product by the kidneys. Increased serum creatinine levels usually indicate impairment of renal function. It is commonly measured to evaluate kidney function and muscle metabolism.
PRINCIPLE
Creatinine in alkaline medium reacts with picric acid to form an orange coloured complex.
The intensity of colour formed is directly proportional to the amount of creatinine present in the sample.
REAGENTS
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Creatinine Reagent
-
Creatinine Standard: 2 mg/dL
MATERIALS REQUIRED BUT NOT PROVIDED
Clean dry glassware
Micropipettes & tips
Laboratory timer
Colorimeter / Spectrophotometer
PREPARATION OF REAGENT & STABILITY
The reagent kit is stable at 2–8°C till expiry date.
Once opened, store reagent properly.
PROCEDURE
| Tube | Reagent | Sample | Standard |
|---|---|---|---|
| Blank | 1.0 ml | – | – |
| Standard | 1.0 ml | – | 100 µl |
| Test | 1.0 ml | 100 µl | – |
Mix well and record absorbance of test (AT) and standard (AS) at 510 nm.
CALCULATION
For Serum
Creatinine (mg/dL) =
For Urine
Creatinine (mg/dL) =
NORMAL VALUES
Serum: Male 0.6 – 1.5 mg/dL, Female 0.4 – 1.4 mg/dL
Urine: Male 15 – 20 mg/kg/day, Female 8 – 15 mg/kg/day
PRECAUTIONS
Do not freeze reagents
Bring reagents to room temperature before use
Use clean and dry glassware
Follow standard laboratory safety precautions