SUMMERY:-  

Serum Glutamate Oxaloacetate Transaminase (SGOT), also called as Aspartate aminotransferase (AST), belongs to the transferase class of enzymes. This enzyme shows high levels of activity in the heart, liver, skeletal muscles and kidneys. Since its level seems to be increasing enormously following Myocardial Infarction (MI), it can be used as supporting evidence in the diagnosis of MI (Especially 20-36 hrs after MI). Elevated levels are also seen in Viral / Toxic Hepatitis, Hepatic and Cardiac Necrosis, Muscular Dystrophy and Pulmonary Embolism.

AIM:-

Alanine aminotransferase (GPT) catalyzes the transfer of the amino group from alanine to oxoglutarate with the formation of glutamate and pyruvate.

L-Aspartate + 2-Oxogluatarate                      ^GPT            Oxaloacetate +L-Glutamate

     Oxaloacetate + 2,4 DNPH                                              Brownish red colored complex

SGOT (AST) catalyses the transfer of amino group from Aspartic acid to 2- Oxoglutarate to form Oxaloacetate and L-Glutamate. The Oxaloacetate thus formed reacts with 2,4 Dinitrophenyl Hydrazine (2,4 DNPH) to form a corresponding Hydrazone, a brownish red colored complex in an alkaline medium.
The color intensity is directly proportional to the SGOT concentration in the serum and is measured photometerically at 505 nm (490- 546).

 REQUIREMENTS:-

·        Four test-tube,

·        Colorimeter,

·        SGOT Reagent Kit,

·        Incubator,

·        Cuvette,

·        Pipette etc.

·        Fresh Serum

PROCEDURE:-

1.      Pipette into test tubes labeled as Blank, Calibrator, Control, Test and proceed as per given below

 1.      Mix and incubate at 37ºC for 60 minutes.

1.      Mix and incubate at 37ºC for 20 minutes.

1.      Read absorbance of all the tubes against distilled water at 505 nm
(490-546).